Nucleotide sequence of a gene for 5S ribosomal RNA from Pseudomonas aeruginosa.
نویسندگان
چکیده
SEQUENCE AND ITS ORIGIN: A 4.5 kb fragment of DNA derived from a library of BamHl fragments of Pseudomonas aeruginosa strain K (ATCC 25102) cloned into plasmid pUC9 was shown by hybridisation with P-labelled 16S and 23S ribosomal RNAs to represent part of a ribosomal RNA operon from that organism. The fragment has been sequenced by the chain termination method (1) after fragmentation and subcloning into the sequencing vectors Ml3mp8 and Ml3mp9. Both strands were sequenced at least three times. A sequence corresponding to 5S ribosomal (nucleotides 7-126) RNA was identified by homology with the corresponding sequence from Escherichia coli (2).
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 16 6 شماره
صفحات -
تاریخ انتشار 1988